Fluorescent metal ion indicators with large stokes shifts
AssigneeLife Technologies Corporation
InventorsKyle Gee, Vladimir Martin
Life of this patent
Estimating the term…
Abstract
The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
Claims12
A compound for the detection of metal ions wherein a detectable response is modulated by photoinduced electron transfer (PET), wherein the compound comprises a fluorescent protein that is covalently bonded to the metal chelating moiety by a linker,
- —(CR2)nNR′—, wherein R and R′ are independently hydrogen, alkyl, or substituted alkyl and n is 1-10;
- or —(CR2)n— wherein R is alkyl or substituted alkyl and n=1-10;
- or (CH2)p wherein p is 2-10.
A compound for the detection of metal ions, wherein the compound comprises:
- a metal chelating BAPTA moiety;
- and
- a fluorescent protein that is covalently bonded to the metal chelating moiety by a linker,
- —(CR2)nNR′—, wherein R and R′ are independently hydrogen, alkyl, or substituted alkyl and n is 1-10;
- or —(CR2)n— wherein R is alkyl or substituted alkyl and n=1-10;
- or (CH2)p wherein p is 2-10.
Dependencies are inferred from the claim text.