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US 9,845,496 B2Granted
Multiplexed digital assay with specific and generic reporters
AssigneeBio-Rad Laboratories, Inc.
InventorsJohn F. Regan, Svilen S. Tzonev, Yann Jouvenot, Claudia Litterst, Dianna Maar, Geoff McDermott
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Abstract
Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.
Claims10
A composition for performing a multiplexed digital assay, comprising:
- an emulsion including droplets, each droplet including a portion of a same aqueous phase and containing (i) a generic reporter that is sensitive to amplification of a first target and a second target and (ii) a specific reporter that is sensitive to amplification of the second target but not the first target;
- wherein each droplet includes a complete set of reagents for amplification of each target, if present in the droplet,
- wherein each droplet of a subset of the droplets contains the first target but not the second target, and
- wherein each droplet of a subset of the droplets contains the second target but not the first target.
1 more element
Dependencies are inferred from the claim text.