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US 9,845,493 B2Granted
Tunable fluorescence using cleavable linkers
AssigneeAgency for Science, Technology and Research
InventorsSydney Brenner, Yin Nah Teo, Pee Mei Tan
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Abstract
The invention relates to cleavable chemistry in general, and in particular, to tunable fluorescence using cleavable linkers present in fluorochrome-quencher conjugates.
Claims23
Fluorochrome-quencher conjugate having the structure
- F-L1-B-L2-Q
- wherein
- F is at least one fluorochrome;
- Q is at least one quenching molecule for the at least one fluorochrome, or is another fluorochrome F, or is another fluorochrome other than F;
- L1 is at least one first cleavable linker molecule;
- L2 is at least one second cleavable linker molecule, wherein said L1 and L2 are different so that fluorophores can be selectively turned on upon specific cleavage of said L1 and L2;
- and
- B is a linking moiety or a target molecule of interest;
- wherein L2 is directly attached to Q via amide coupling to form part of a modular system of the conjugate.
6 more elements
Dependencies are inferred from the claim text.