Selective chromogenic medium
AssigneesSzegedi TudomáNyegyetem, SOLVO Biotechnológiai ZRt.
InventorsCsaba Vágvölgyi, Ilona Pfeiffer, János Márky-Zay
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Abstract
The invention deals with chromogenic media which are suitable for the selective growth and identification of one or more species of yeast. The subject of the invention is the method that enables us to identify and determine the cell count of Brettanomyces/Dekkera and Zygosaccharomyces yeasts. Besides, the subjects of invention are also the use of the method in wine and/or food industry and the stocks for conducting the experiment.
Claims19
A chromogenic medium suitable for the selective growth and detection of one or more yeast(s) from a yeast of the Brettanomyces and/or Dekkera genera, a yeast of the Zygosaccharomyces genus, and/or a yeast of the Lachancea genus, said medium comprising
- at least a nutrient suitable for the feeding and/or growing of said one or more yeast(s) to be detected,
- an agent capable of inhibiting the growth of Saccharomyces species, and
- a chromogenic stain, wherein the chromogenic stain is the combination of the following dyes, said combination being chromatic under visible light:
- multiple type(s) of substituted and/or unsubstituted bis-3,7 diaminophenothiazines comprising a mixture of methylene blue and Azure B, and
- one or multiple type(s) of substituted fluorescein(s),
- wherein the substituted fluorescein is selected from the group consisting of eosin Y, eosin B and any mixture thereof.
Dependencies are inferred from the claim text.