Transcriptome transfer produces cellular phenotype conversion
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel
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Abstract
The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
Claims19
A method of effecting phenotype conversion in a cell, said method comprising introducing phenotype-converting nucleic acid of a first cell having a phenotype into a second cell having a phenotype, wherein the phenotype-converting nucleic acid comprises at least one RNA, wherein the phenotype of said first cell is different from the phenotype of said second cell, wherein the second cell is pre-treated with a transcription inhibitor before it is transfected, wherein said transfected phenotype-converting nucleic acid causes the phenotype of said second cell to change to the phenotype of said first cell, and wherein said phenotype-converting nucleic acid is locally administered to said second cell in vivo.
Dependencies are inferred from the claim text.