Plant regulatory elements and uses thereof
AssigneeMonsanto Technology LLC
InventorsStanislaw Flasinski, Barrett C. Foat, Mohammed Oufattole, Randall W. Shultz, Xiaoping Wei, Wei Wu, Shiaw-Pyng Yang
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Abstract
The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
Claims14
A DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- b) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- c) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic plant cell comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- b) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- c) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic plant, or part thereof, comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- b) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- c) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A transgenic seed comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- b) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- c) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A method of producing a commodity product comprising:
- a) obtaining a transgenic plant or part thereof comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- 1) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- 2) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- 3) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule;
- and
- b) producing the commodity product from the transgenic plant or part thereof.
A commodity product comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- b) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- c) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.
A method of expressing a heterologous transcribable polynucleotide molecule comprising:
- a) obtaining a transgenic plant comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
- 1) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
- 2) a sequence comprising any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
- and
- 3) a fragment of any one of SEQ ID NOs:
- 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO:
- 12, and exhibiting promoter activity;
- wherein said DNA molecule 1s operably linked to the heterologous transcribable polynucleotide molecule;
- and
- b) cultivating said transgenic plant, wherein the heterologous transcribable polynucleotide is expressed.
Dependencies are inferred from the claim text.