Targeted gene deletions for polysaccharide slime formers
AssigneeCP Kelco U.S., Inc.
InventorsNancy E. Harding, Yamini N. Patel, Russell J. Coleman
Life of this patent
Estimating the term…
Abstract
The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R, M or N Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccharide production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
Claims7
A Sphingomonas mutant strain comprising a mutation in gelM gene, wherein said Sphingomonas mutant strain has been genetically engineered to eliminate expression of gene gelM, wherein said mutant strain produces gellan, wherein said gellan is a slime form polysaccharide, and wherein said gellan has improved rheology, as compared to gellan naturally produced by the wild-type Sphingomonas strain ATCC 31461.
Dependencies are inferred from the claim text.