Expression vector
AssigneeFujifilm Diosynth Biotechnologies UK Limited
InventorsBhupendra Vallabh Kara, Christopher David John Lennon
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Abstract
An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.
Claims16
A prokaryotic expression vector comprising a prokaryotic promoter operably linked to a heterologous polynucleotide encoding a target polypeptide operably linked to a eukaryotic secretion leader, the eukaryotic secretion leader encoded by the polynucleotide being a signal peptide having an amino acid sequence at least 90% identical to SEQ ID NO:
- 1.
A method for producing a target polypeptide which comprises:
- a) culturing a prokaryotic host cell comprising the prokaryotic expression vector comprising a prokaryotic promoter operably linked to a heterologous polynucleotide encoding a target polypeptide operably linked to a eukaryotic secretion leader, the eukaryotic secretion leader encoded by the polynucleotide being a signal peptide having a sequence at least 90% identical to SEQ ID NO:
- 1, thereby to express the target polypeptide, and
- b) recovering the target polypeptide.
Dependencies are inferred from the claim text.