Method of obtaining high purity stem cells from tissue

A method for chitosan matrix purification by absorbing oil drops to obtain high purity stem cells from tissue, consisting the steps of:

• (a) providing an oil drops-containing cell mass isolated from a tissue through a cell mesh of pore diameter of 70 μm;
• (b) providing a filter device including:
• a cylinder structure having an inlet and an outlet, the outlet is configured below the inlet, and the oil drops-containing cell mass flows into the filter device via the inlet;
• and
• a content, configured inside the cylinder structure between the inlet and the outlet for separating the oil drops from the oil drops-containing cell mass by absorbing oil drops and allowing an oil drops-free cell mass to pass through the outlet, and the diameter of pores of the content is 100 μm to 150 μm;
• (c) recovering the oil drops-free cell mass;
• (d) culturing the oil drops-free cell mass on a polymeric film wherein target stem cells of the oil drops-free cell mass conjugate into a spheroid cell population;
• and
• (e) collecting the spheroid cell population from the polymeric film to obtain high purity target stem cells;
• wherein the content in step (b) is formed by a matrix, the formation of the matrix comprises dissolving chitosan in an acidic solution to form a mixture, injecting the mixture into the cylinder structure and allowing the mixture to solidify therein;
• and the matrix is made of at least one material selected from the group consisting of chitosan, sulfonated chitosan, and chitosan-heparin, the matrix absorbs oil drops from the oil drops-containing cell mass to obtain oil drops-free cell mass, and the high purity stem cells have an at least 3-fold increase in number after incubation for 7 days without transferring to a tissue culture plate.