Multipotent progenitor cell derived from adipose tissue

A method for increasing purity of Islet-1 expressing adipose tissue-derived multipotent progenitor cell in a culture medium from an adipose tissue, comprising:

• (a) slicing and digesting adipose tissue to make an adipose cell extract of separated cells of adipose tissue-derived cell population;
• (b) clarifying the separated cells from naturally occurring tissue and digestion products by centrifugation;
• (c) selectively removing erythrocytes from the clarified adipose tissue-derived cell population by the density method;
• (d) culturing in the presence of feeder cells and then washing the erythrocyte-free clarified adipose tissue-derived cell population;
• and
• (e) selectively removing vascular endothelial cells from the cultured and washed erythrocyte-free clarified adipose tissue-derived cell population by treatment with EDTA
• thereby forming a tissue culture enriched in Islet-1 expressing adipose tissue-derived multipotent progenitor cell population with respect to erythrocytes and respect to vascular endothelial cells, and having a low fibroblast contamination comprising a 23 percent fibroblast cell contamination.