Maintenance of genomic stability in cultured stem cells
AssigneeCedars-Sinai Medical Center
InventorsEduardo Marbán, Tao-Sheng Li
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Abstract
The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and/or augment the functionality of such cells for cellular therapies.
Claims8
A method for reducing the incidence of karyotypic abnormalities in cardiac stem cells for use in the repair or regeneration of cardiac tissue, the method comprising:
- culturing cardiac stem cells in a culture media supplemented with an antioxidant composition,
- wherein said antioxidant composition comprises glutathione present in a concentration ranging from 5 to 20 μM, vitamin C present in a concentration ranging from 5 to 20 μM, and vitamin E present in a concentration ranging from 5 to 20 μM.
Dependencies are inferred from the claim text.