Composition containing complex cytokines derived from EBV-infected B cells for inducing the maturation of dendritic cells

A method of producing dendritic cells, the method comprising:

• a) isolating monocytes from human peripheral blood mononuclear cells (PBMCs);
• b) generating a culture supernatant consisting essentially of cytokines interleukin-1 A, interleukin-1B, interleukin-4, interleukin-5, interleukin-6, interleukin-8, interleukin-10, interleukin-12, interleukin-17A, and Granulocyte-macrophage colony-stimulating factor (GM-CSF);
• wherein the culture supernatant is generated by:
• (i) incubating Epstein-Barr virus (EBV)-infected B cells with an anti-CD150 antibody to stimulate CD150 expressed on the surface of the EBV-infected B cells;
• or
• (ii) incubating Epstein-Barr virus (EBV)-infected B cells with a measles virus core protein selected from the group consisting of MV1 (SEQ ID NO:
• 34) and MV2 (SEQ ID NO:
• 35) to stimulate CD150 expressed on the surface of the EBV-infected B cells;
• wherein the stimulation of CD150 on the surface of the EBV-infected B cells induces the secretion of interleukin-1 A, interleukin-1B, interleukin-4, interleukin-5, interleukin-6, interleukin-8, interleukin-10, interleukin-12, interleukin-17A, and Granulocyte-macrophage colony-stimulating factor (GM-CSF) into the supernatant;
• and
• c) differentiating the monocytes into dendritic cells by culturing the monocytes with the supernatant obtained from step b) above to produce the dendritic cells.