Multilayered cell culture apparatus
AssigneeCorning Incorporated
InventorsGregory Roger Martin, Allison Jean Tanner
Life of this patent
Estimating the term…
Abstract
A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
Claims23
A cell culture vessel comprising:
- a top plate;
- a bottom tray opposed to the top plate;
- and
- a plurality of vertically spaced and aligned cell growth chambers that are between the top plate and the bottom tray, each cell growth chamber defined by a gas permeable, liquid impermeable membrane bottom surface and a top surface, said top and bottom surfaces attached to a pair of side walls and an end wall, wherein the top surface of a first cell growth chamber and the gas permeable, liquid impermeable membrane bottom surface of a second cell growth chamber adjacent the first cell growth chamber define a tracheal space, said tracheal space being in gaseous communication with the external environment through openings between the side walls and end walls of adjacent cell growth chambers.
A tissue cell culture vessel flask comprising:
- a top plate;
- a bottom tray opposed to the top plate;
- side walls and an end wall that are between the top plate and bottom tray;
- and
- at least two vertically spaced and aligned cell growth chambers within the cell culture vessel, each cell growth chamber defined by a gas permeable, liquid impermeable membrane bottom surface and a top surface, said top and bottom surfaces attached to the side walls and the end wall of the cell culture vessel, wherein the top surface of a first cell growth chamber and the gas permeable, liquid impermeable membrane bottom surface of a second cell growth chamber adjacent the first cell growth chamber define a tracheal space, said tracheal space being in gaseous communication with the external environment through an opening in the side walls and the end wall of the cell culture vessel, said opening being continuous around the side walls and the end wall of the cell culture vessel interrupted by supports.
Dependencies are inferred from the claim text.