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US 9,845,347 B2Granted
Alkaline feed
AssigneeHoffman-La Roche Inc.
InventorChristian Schantz
Life of this patent
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Abstract
A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.
Claims8
A method for producing a polypeptide comprising:
- a) cultivating an Escherichia coli cell comprising a nucleic acid encoding the polypeptide, the peptide comprising an apolipoprotein A1 having an amino acid sequence selected from SEQ ID NO:
- 01, 02, 34, and 35,
- b) adjusting the pH value during the cultivating with an alkaline solution comprising an amino acid selected from aspartate, aspartic acid, glutamine, glutamic acid, histidine, isoleucine, leucine, methionine, phenylalanine, tryptophan and tyrosine,
- c) recovering the polypeptide from the cell and thereby producing the polypeptide,
- wherein the amino acid has a concentration in the alkaline solution of 30 g/l or more,
- wherein the alkaline solution is an ammonia solution of 10% (w/v) or more, and
- wherein the dry cell weight of the cultivated bacterial cell is at one point in the cultivating at least 20 g/l.
4 more elements
Dependencies are inferred from the claim text.