Method of purifying an antibody
AssigneeSynthon Biopharmaceuticals B.V.
InventorsBastiaan Pieter Arjan Kokke, Everdina Josephina Wilhelmina Wijk-Basten Van, Thomas Antonius Bernardus Beijer De, Maria Marzá Pérez, Michel Hendrikus Maria Eppink
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Abstract
A method of purifying an antibody composition comprises application of anion exchange chromatography late in the purification process. An ultrafiltration/diafiltration-purified antibody composition is subjected to anion exchange chromatography (AEX) to form a pharmaceutically-pure antibody composition.
Claims22
A method of purifying an antibody composition, wherein the antibody is produced in eukaryotic cells, which comprises subjecting a UF/DF-purified antibody composition to anion exchange chromatography (AEX) to form a pharmaceutically-pure antibody composition, wherein said anion exchange chromatography is the last chromatographic purification step and is performed directly after the final UF/DF step.
A method of purifying an antibody composition, which comprises:
- subjecting an antibody eukaryotic cell culture harvest to affinity chromatography to capture a crude antibody composition;
- subjecting the crude antibody composition to at least one polishing step to form a partially-purified antibody composition;
- subjecting the partially-purified antibody composition to a UF/DF step to form a UF/DF-purified antibody composition;
- and
- subjecting the UF/DF-purified antibody composition to anion exchange chromatography (AEX) to form a pharmaceutically-pure antibody composition,
- wherein said anion exchange chromatography is the last chromatographic purification step and is performed directly after the final UF/DF step.
Dependencies are inferred from the claim text.