Class of near infrared optical probes for biological applications
AssigneeThe University of Akron
InventorsYi Pang, Yongqian Xu
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Abstract
2,5-Bis(benzoxazol-2′-yl)benzene-1,4-diol derivatives (Zinhbo derivatives) are used to detect zinc ions and have particular application in vivo and in vitro. Zinhbo derivatives upon excitation give a florescence response emission that can be used to determine the presence of zinc cation in solution. Zinhbo derivatives complexed with zinc cations upon excitation can produce a florescence response emission in the visible and near infrared range. Zinhbo derivatives complexed with zinc cations exhibit a large stoke shift between the excitation and emission wavelengths.
Claims13
A method for detecting the presence of zinc ions in solution, the method comprising the steps of:
- contacting a solution with a Zinhbo derivative defined by the formula:
- where R1 is an electron withdrawing group or a hydrogen atom;
- R2, R3, and R4 are each individually selected from the group consisting of a hydrogen atom, an electron withdrawing group, and an electron donating group;
- and R5 is a chelator group capable of forming a complex with a zinc cation;
- exciting the solution with an excitation wavelength;
- and
- measuring a fluorescence response emission in at least one of the visible or near infrared ranges;
- wherein the fluorescence response emission of the Zinhbo derivative corresponds to a Zinhbo derivative complexed with a zinc ion indicating the presence of zinc ions in the solution.
A method for detecting the presence of zinc ions, the method comprising the steps of:
- contacting living cells or a living organism with a Zinhbo derivative;
- exciting the living cells or the living organism with an excitation wavelength;
- and
- measuring a fluorescence response emission in at least one of the visible or near infrared range;
- wherein the fluorescence response emission of the Zinhbo derivative corresponds to a Zinhbo derivative complexed with a zinc ion indicating the presence of zinc ions in the living cells or living organism;
- where the Zinhbo derivative is defined by the formula:
- where R1 is an electron withdrawing group or a hydrogen atom:
- R2, R3, and R4 are each individually selected from the group consisting of a hydrogen atom, an electron withdrawing group, and an electron donating group;
- and R5 is a chelator group capable of forming a complex with a zinc cation.
Dependencies are inferred from the claim text.