Protein or peptide printing method, protein array or peptide array, and functional protein or functional peptide identification method

A protein or peptide printing method, comprising:

• (a) preparing a mixture of:
• a plurality of types of DNA beads in each of which a single type DNA is immobilized on the beads through avidin-biotin or streptavidin-biotin binding, a cell-free protein synthesis system, a biotinylated complex of puromycin and nucleotide, in an engraved plate composed of microscopic grooves having a specific opening shape, a diameter of the microscopic grooves being 1 to 2 times a diameter of the beads, and a depth of the microscopic grooves being 1 to 2 times a diameter of the beads,
• (b) superimposing an avidin-labeled substrate or a streptavidin-labeled substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and
• (c) synthesizing the protein or peptide from the DNA using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the avidin-labeled substrate or the streptavidin-labeled substrate along the specific opening shapes of the microscopic grooves.

A functional protein or functional peptide screening method, comprising:

• (a) preparing a mixture of:
• a plurality of types of DNA beads in each of which a single type DNA is immobilized on the beads, and a cell-free protein synthesis system, in an engraved plate composed of microscopic grooves having a specific opening shape, a diameter of the microscopic grooves being 1 to 2 times a diameter of the beads, and a depth of the microscopic grooves being 1 to 2 times a diameter of the beads,
• (b) superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves,
• (c) synthesizing the protein or peptide from the DNA using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate, thereby manufacturing a protein or peptide array, and
• (d) carrying out functional screening using the protein or peptide array.