Method for preparing in situ-formed hydrogel using enzyme-immobilized support, and biomedical use thereof

A method of preparing a hydrogel, the method comprising:

• modifying a support with a catechol derivative, wherein the catechol derivative is selected from the group consisting of 3,4-dihydroxy hydrocinnamic acid, dopamine, chloroacetylcatechol, aminomethylcatechol, deoxyepinephrine, dihydroxybenzohydrazine, caffeic acid phenethylester, and hirsutenone;
• binding an enzyme onto a functional group of the catechol derivative, after modifying the support, to prepare an enzyme-immobilized support;
• cross-linking a polymer to form the hydrogel in the presence of hydrogen peroxide and in the presence of the enzyme-immobilized support, wherein the polymer comprises a phenol or aniline functional group side chain;
• and
• separating the cross-linked polymer away from the enzyme-immobilized support by extruding the cross-linked polymer through a filter to obtain the hydrogel in which the filter restricts the enzyme-immobilized support from extruding through the filter.

A method of preparing a hydrogel, the method comprising:

• modifying a support with a silane derivative, wherein the silane derivative is selected from the group consisting of 3-aminopropyl triethoxysilane, trimethoxy (7-octane-1-yl) silane, vinyltrimethoxysilane, 3-trimethoxysilyl-1-propanethiol, 3-aminopropyl trimethoxysilane, aminoethyl trimethoxysilyl propylamine, triethoxyvinylsilane, isocyanatopropyl triethoxysilane, cyanoethyl triethoxysilane, mercaptopropyl triethoxysilane, and triethoxysylylpropyl diethanolamine;
• binding an enzyme onto a functional group of the silane derivative, after modifying the support, to prepare an enzyme-immobilized support;
• cross-linking a polymer, to form the hydrogel, in the presence of hydrogen peroxide and in the presence of the enzyme-immobilized support, wherein the polymer comprises a phenol or aniline side chain functional group;
• and
• separating the cross-linked polymer away from the enzyme-immobilized support by extruding the cross-linked polymer through the filter to obtain the hydrogel in which the filter restricts the enzyme-immobilized support from extruding through the filter.