Indocyanine compound, synthesis method and purification method thereof, diagnostic composition using the indocyanine compound, and device for measuring biokinetics and device for visualizing circulation using the diagnostic composition

A method of visualizing at least one of kidney, ureter, bladder, and urethra, comprising:

• intravenously administering to a living body a composition comprising water and at least one of cyclodextrin-bonded indocyanine cations of formulas (15), (16), (19), (20), (21), (23), (24) and (25):
• applying excitation light to the at least one of cyclodextrin-bonded indocyanine cations in the living body;
• and
• visualizing the at least one of kidney, ureter, bladder and urethra based on distribution data of the at least one of cyclodextrin-bonded indocyanine cations in the living body.

A method of visualizing at least one of blood vessel, lymph vessel, brain, eye, stomach, breast, esophagus, skin, kidney, ureter, bladder, urethra, lung and heart, comprising:

• administering to a living body a solution comprising water and at least one of cyclodextrin-bonded indocyanine cations of formulas (15), (16), (19), (20), (21), (23), (24) and (25):
• applying excitation light to the at least one of cyclodextrin-bonded indocyanine cations in the living body;
• obtaining distribution state data of the at least one of cyclodextrin-bonded indocyanine cations in the living body by two-dimensionally acquiring intensity of a fluorescence emitted by the cyclodextrin-bonded indocyanine cation in the living body and two-dimensionally acquiring an intensity of light other than the fluorescence emitted by the cyclodextrin-bonded indocyanine cation excited by the excitation light;
• obtaining morphological data of a visualized part of the living body by two-dimensionally acquiring an intensity of light different from the fluorescence emitted by the cyclodextrin-bonded indocyanine cation, and
• displaying a distribution state of the cyclodextrin-bonded indocyanine cation in the part of the living body by overlapping the morphological data obtained by the morphological with the distribution state data obtained by the fluorescence imager.

A method of detecting a visualized image of at least one of an organ, a body fluid, and a vessel in a living body by near-infrared fluorescence, comprising:

• administering to the living body a composition comprising water and at least one of cyclodextrin-bonded indocyanine cations of formulas (15), (16), (19), (20), (21), (23), (24) and (25):
• applying excitation light to the at least one of cyclodextrin-bonded indocyanine cations in the living body;
• and
• detecting the visualized image of the at least one of the organ, the body fluid, and the vessel based on distribution data of the at least one of cyclodextrin-bonded indocyanine cations which has been excited by the excitation light in the living body.