Methods and compositions employing immunogenic fusion proteins
AssigneeSt. Jude Children's Research Hospital
InventorsElaine Tuomanen, Elizabeth R. Mann
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Abstract
Compositions and methods are provided for the prevention and treatment of bacterial infections, including pneumococcal infections. Compositions provided herein comprise a variety of immunogenic fusion proteins, wherein at least one polypeptide component of a given fusion protein comprises a CbpA polypeptide and/or a cytolysoid polypeptide, or an active variant or fragment thereof. Methods are provided for the prevention and treatment of bacterial infections, including pneumococcal infections by employing the various immunogenic fusion proteins having at least one polypeptide component comprising a CbpA polypeptide and/or a cytolysoid polypeptide, or an active variant or fragment thereof.
Claims10
An isolated polynucleotide that encodes a fusion protein, said fusion protein comprising
- a) a first polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 4, wherein said first polypeptide forms a loop conformation and is immunogenic,
- b) a second polypeptide, fused in frame to said first polypeptide, said second polypeptide comprises at least one T cell epitope (TCE), and
- c) a third polypeptide comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:3 or SEQ ID NO:4, wherein said third polypeptide forms a loop conformation and is immunogenic;
- and wherein said fusion protein comprises said first, said second and said third polypeptide in the following order:
- said first polypeptide operably linked to said second polypeptide operably linked to said third polypeptide and wherein said second polypeptide is heterologous to said first and said third polypeptides.
Dependencies are inferred from the claim text.