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US 9,844,586 B2Granted
Method for producing an antitumoral vaccine based on surface endothelial cell antigens
OwnerPetr Genrievich Lokhovinventor
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OwnerPetr Genrievich Lokhovinventor
Estimating the term…
Accordingly to the method of the preparing of the tumor vaccine with the use of endothelial cells, live endothelial cells are treated with a protease at mild (non-deadly for cells) conditions, the splitted surface antigens are collected, the treatment of live endothelial cells is repeated after intervals which are necessary for the recovery of the surface antigens by the cells, surface antigens are accumulated until their necessary quantity is reached, the quality of the vaccine is controlled thereafter. The technical result obtained with the use of this invention consists in the enhancement of the efficiency of oncological disease treatment due to the damage of the tumor vessels caused by overcoming of the immune tolerance of organism to the endothelial cells (EC) of tumor vessels. Here one means the overcoming of immune tolerance namely to activated EC, which allows to damage mainly to the tumor vessels by the immune system.
A method of preparing a tumor cell vaccine comprising treating live, activated endothelial cells with trypsin having activity of at least 3000 U/mg and concentration from 0.00001% to 0.5% under conditions non-deadly for endothelial cells, and collecting released cell surface antigens, wherein the endothelial cells are activated in vitro by adding a conditioned growth medium from a tumor cell culture.
A method of preparing a tumor cell vaccine comprising treating live, activated endothelial cells with trypsin having a concentration from 0.00001% to 0.5% under conditions non-deadly for endothelial cells, and collecting released cell surface antigens, wherein the endothelial cells are activated in vitro by adding a conditioned growth medium from a tumor cell culture.
A method of preparing a tumor vaccine comprising treating live, activated endothelial cells with a protease at conditions non-deadly for endothelial cells, and collecting the released cell surface antigens, wherein the endothelial cells are activated in vitro by adding a conditioned growth medium from a tumor cell culture.
A method of preparing a tumor vaccine comprising treating live, activated endothelial cells with a protease at conditions non-deadly for endothelial cells, and collecting the released cell surface antigens, wherein the activated endothelial cells are isolated from tumor vessels and maintained in an activated state during cultivation prior to the treatment with the protease by the addition of activating substances into the growth medium, wherein the activating substances are at least one of conditioned growth medium from a tumor cell culture, and vessel endothelium growth factor (VEGF).
Dependencies are inferred from the claim text.