Polyvalent RNA-nanoparticle compositions
AssigneeNorthwestern University
InventorsChad A. Mirkin, David A. Giljohann, Dwight S. Seferos, Andrew E. Prigodich, Pinal C. Patel
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Abstract
The present invention concerns nanoparticles functionalized with duplex RNA for a variety of uses, including but not limited to gene regulation. More specifically, the disclosure provides a new strategy for conjugating RNA to a nanoparticle to achieve increased stability and activity.
Claims23
An in vivo method of regulating expression of a target polynucleotide comprising the step of hybridizing the target polynucleotide with a domain of a nanoparticle composition to form a substrate site for a polypeptide, the nanoparticle composition comprising a duplex, the duplex comprising:
- (a) a ribonucleic acid (RNA) polynucleotide functionalized to the nanoparticle and comprising a sequence that is identical to the target polynucleotide;
- and
- (b) an additional polynucleotide having a sequence that forms the duplex with the RNA polynucleotide under conditions appropriate to form the duplex, the duplex providing a polypeptide interaction site;
- the additional polynucleotide having at least one domain sufficiently complementary to a sequence in the target polynucleotide to permit hybridization of the domain to the target polynucleotide under appropriate conditions, wherein
- the duplex is located distal and not proximal to the nanoparticle, and the distance of the duplex from the nanoparticle is equivalent to at least 10 nucleotides.
Dependencies are inferred from the claim text.