Tumor vaccine and method for producing the same
AssigneeHubei Soundny Biotechnology Co., Ltd.
InventorBo Huang
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Abstract
The invention provides a tumor vaccine and method for producing the same. The tumor vaccine comprises cell vesicles derived from apoptotic tumor cells and an adjuvant. The invention further provides a preparation method of the tumor vaccine, comprising the steps of using the UV to irradiate the tumor cells to induce apoptosis, and collecting the cell vesicles released from the apoptotic tumor cells and then mixing the cell vesicles with the adjuvant to form the tumor vaccine. The tumor vaccine provided by the invention contains a broad and comprehensive tumor antigen spectrum, the defect that the existing tumor vaccine cannot have the killing capacity against the broad tumor cells can be overcome, and at the same time the tumor vaccine has good use safety and immune targeting property.
Claims6
A tumor vaccine, comprising microparticles, wherein the microparticles are cell vesicles derived from apoptotic tumor cells, and an adjuvant, wherein the microparticles are generated by cellular membrane encapsulating cytosolic contents, and wherein the particle size of the microparticles is 100-1000 nm;
- wherein the microparticles are obtained by:
- centrifuging cell culture medium that contains apoptotic tumor cells and collecting a first supernatant;
- centrifuging the first supernatant at a centrifugal force of not more than 14000 g so as to remove cells and debris, and collecting a second supernatant;
- further centrifuging the second supernatant at a centrifugal force of 14,000 g-100,000 g to obtain the microparticles.
Dependencies are inferred from the claim text.