Human lambda light chain mice
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn Macdonald, Sean Stevens, Cagan Gurer, Andrew J. Murphy, Karolina A. Meagher
Life of this patent
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Abstract
Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided.
Claims30
A mouse whose genome comprises:
- (a) a first light chain allele comprising human Vλ and Jλ gene segments operably linked to an endogenous mouse Cκ gene such that the mouse expresses an immunoglobulin light chain that comprises a human λ variable domain sequence fused with a mouse κ constant domain;
- and
- (b) a second light chain allele comprising human Vκ and Jκ gene segments operably linked to an endogenous mouse Cκ gene such that the mouse expresses an immunoglobulin light chain that comprises a human κ variable domain sequence fused with a mouse κ constant domain.
A mouse whose genome comprises:
- (a) a first light chain allele comprising human Vλ and Jλ gene segments operably linked to an endogenous mouse Cκ gene at an endogenous κ locus such that the mouse expresses an immunoglobulin light chain that comprises a human λ variable domain sequence fused with a mouse κ constant domain;
- and
- (b) a second light chain allele comprising Vκ and Jκ gene segments operably linked to an endogenous mouse Cκ gene at an endogenous κ locus such that the mouse expresses an immunoglobulin light chain that comprises a human κ variable domain sequence fused with a mouse κ constant domain.
Dependencies are inferred from the claim text.