Systems and methods for autologous biological therapeutics

A cell concentrating system comprising:

• a blood separation component having a blood sample input and having a centrifuge to separate the blood sample into a red blood cell fraction and a plasma fraction, the plasma fraction comprising a target cell-rich fraction and a target cell-poor fraction, the blood sample having a volume of between 60 mL and no more than 250 mL;
• a first vessel for storing the target cell-poor fraction separated from the blood sample;
• a second vessel for storing the target cell-rich fraction separated from the blood sample;
• a first valve upstream of the first vessel and the second vessel, and downstream of the blood separation component;
• a second valve for providing selective fluid communication between the first vessel and the second vessel;
• a concentration and flow logic and control component comprising a concentration measurement component, a partial separation component, and a controller, the concentration measurement component comprising at least one of a fluorescent activated cell sorting component and an optical sensor, the partial separation component comprising a microfluidic channel device for separating particles within the blood sample and a light scattering device for measuring a number of particles in the blood sample, and the controller comprising a processor and memory configured to:
• control the first valve to remove the target cell-poor fraction to the first vessel and to remove the target cell-rich fraction to the second vessel;
• determine, via the controller, a first volume of the target cell-poor fraction in the first vessel to mix with the target cell-rich fraction in the second vessel in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range for studying or defining a dose-response relationship in a patient, wherein the first volume is determined by the controller via information from a flow meter or from a calculation based upon a known concentration of the blood sample and a known volume of the blood sample that is in the memory of the controller;
• determine, via the concentration measurement component, whether the target cell-rich concentrate has a concentration of target cells within the target concentration ranges wherein the concentration is determined by the controller via information from the flow meter and a hematology analyzer;
• control, via the controller, the second valve to transfer the first volume of the target cell-poor cell rich fraction from the first vessel to the second vessel to form the target cell-rich concentrate;
• and
• wherein the target concentration range is between 1.0 and 1.5×106 target cells/uL.