Conjugation of Staphylococcus aureus type 8 capsular polysaccharides
AssigneeGlaxosmithkline Biologicals SA
InventorsFrancesco Berti, Paolo Costantino, Maria Rosaria Romano
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Abstract
The invention provides a process for preparing a conjugate of a S. aureus type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.
Claims12
A process for preparing a conjugate of a fragment of S. aureus type 8 capsular polysaccharide and a carrier molecule, comprising the steps of:
- (a) depolymerising a S. aureus type 8 capsular polysaccharide by acid hydrolysis under conditions suitable for the depolymerisation, to give a fragment of the S. aureus type 8 capsular polysaccharide having an average molecular mass of between 1 and 500 kDa;
- (b) oxidising the fragment of the type 8 capsular polysaccharide in order to introduce an aldehyde group into at least one saccharide residue in the fragment to have at least one oxidised saccharide residue in the fragment;
- and (c) coupling the fragment having the at least one oxidised saccharide residue to the carrier molecule via the aldehyde group, thereby preparing the conjugate.
Dependencies are inferred from the claim text.