Scanning luminescence light microscope with gratings of luminescence inhibition light and further light

A scanning luminescence light microscope for spatial high resolution imaging a structure in a sample, the structure comprising a luminescent marker, the microscope comprising

• a light source configured to provide luminescence inhibition light and excitation light differing from the luminescence inhibition light and exciting the luminescence marker for emission of luminescence light;
• a light shaping and aligning device including an objective lens and configured to form, from two non-coherent beams of the luminescence inhibition light, by means of two optical gratings, two pairs of coherent partial beams of the luminescence inhibition light and to focus and superimpose the two pairs of partial beams of the luminescence inhibition light by means of the objective so that the two pairs of partial beams of the luminescence inhibition light form two crossing line gratings of the luminescence inhibition light in the area of the sample, each of the two crossing line gratings of the luminescence inhibition light comprising a plurality of intensity minima delimited in one direction so that an intensity distribution of the luminescence inhibition light in the sample comprises a two-dimensional array of local intensity minima which are delimited in at least two directions,
• wherein two non-coherent beams of the excitation light enter the light shaping and aligning device, each together with one of the two beams of the luminescence inhibition light, so that the light shaping and aligning device, from the two non-coherent beams of the excitation light, by means of the optical gratings, forms two pairs of coherent partial beams of the excitation light and focuses and superimposes the two pairs of coherent partial beams of the excitation light by means of the objective so that the two pairs of coherent partial beams of the excitation light form two crossing line gratings of the excitation light in the area of the sample, each of the two crossing line gratings of the excitation light comprising a plurality of intensity maxima delimited in one direction and intensity minima extending in parallel to the local intensity minima of one of the line gratings of the luminescence inhibition light so that an intensity distribution of the excitation light in the sample comprises a two-dimensional array of local intensity maxima and local intensity minima which are each delimited in at least two directions;
• wherein the light shaping and aligning device is configured to align the intensity distribution of the excitation light with regard to the intensity distribution of the luminescence inhibition light in such a way that the local intensity maxima of the excitation light coincide with the local intensity minima of the intensity distribution of the luminescence inhibition light;
• wherein the light shaping and aligning device is configured to move the intensity distributions of the excitation light and the luminescence inhibition light with regard to the sample to scan an area of interest of the sample with the intensity minima of the intensity distribution of the luminescence inhibition light;
• and
• a detector configured to register the luminescence light emitted out of the individual intensity minima of the intensity distribution of the luminescence inhibition light separately for each of the intensity minima and for each position of each of the intensity minima within the sample.