Two-layer analyte sensor
AssigneeVerily Life Sciences LLC
InventorsZenghe Liu, Huanfen Yao
Life of this patent
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Abstract
An analyte sensor and a method for making the analyte sensor are disclosed. In one aspect, the analyte sensor includes a sensing membrane having a crosslinked network with an embedded analyte sensing component. In another aspect, the analyte sensor includes a protective membrane adjacent to the surface of the sensing membrane. The protective membrane can be a crosslinked, hydrophilic copolymer having methacrylate-derived backbone chains of first methacrylate-derived units, second methacrylate-derived units and third methacrylate-derived units. The first and second methacrylate-derived units have hydrophilic side chains, and the third methacrylate-derived units in different backbone chains are connected by hydrophilic crosslinks. A method for making the analyte sensor is also disclosed.
Claims18
An analyte sensor comprising:
- a sensing membrane comprising:
- a crosslinked network comprising one or more proteins and crosslinks, wherein
- the proteins are crosslinked through carbon-nitrogen double bonds between amine group nitrogen atoms on the proteins and carbon atoms on the crosslinks;
- and
- an analyte sensing component embedded in the crosslinked network;
- a protective membrane adjacent to a surface of the sensing membrane, comprising
- backbone chains comprising:
- first methacrylate-derived units, each having a hydrophilic side chain;
- second methacrylate-derived units, each having a hydrophilic side chain comprising one or more alkylene oxide unit;
- and
- third methacrylate-derived units;
- and
- hydrophilic crosslinks between the third methacrylate-derived units in different backbone chains, wherein the hydrophilic crosslinks comprise poly(alkylene oxide).
A method for manufacturing an analyte sensor, the method comprising:
- forming a sensing mixture comprising one or more proteins, a crosslinking agent, and an analyte sensing component, wherein the proteins have one or more amine functional groups;
- depositing the sensing mixture onto a surface of an electrode;
- curing the deposited sensing mixture to provide a sensing membrane;
- forming a copolymer mixture comprising an initiator, a first methacrylate monomer having a hydrophilic side chain comprising one or more alkylene oxide unit, a dimethacrylate monomer, and a second methacrylate monomer having a hydrophilic side chain, wherein the dimethacrylate monomer comprises two terminal methacrylate groups tethered by a hydrophilic linker comprising poly(alkylene oxide);
- depositing the copolymer mixture onto a surface of the sensing membrane;
- and
- curing the deposited copolymer mixture to provide a protective membrane.
Dependencies are inferred from the claim text.