Back to results
US 9,828,622 B2Granted
Photoactivatable antimicrobial agents and therapeutic and diagnostic methods of using same
AssigneeThe General Hospital Corporation
InventorsTayyaba Hasan, Ulysses W. Sallum, Sarika Verma, Gerard Nau
Life of this patent
Estimating the term…
Abstract
The present invention provides photosensitizer compounds for use in detecting beta-lactamase activity. Methods and kits that utilize the photosensitizer compounds of the invention for the detection of, quantitation of, and classification or typing of microbial beta-lactamases.
Claims4
A method of measuring susceptibility of beta-lactamase secreting bacteria in a sample to a beta-lactam antibiotic, wherein, the method comprises:
- (a) contacting the sample with:
- (i) a composition comprising a cephalosporin linker compound linked with two benzophenothiazinium chloride (EtNBS) fluorophores (standard);
- and
- (ii) a composition comprising the beta-lactam antibiotic and the cephalosporin linker compound linked with two EtNBS fluorophores (test sample),
- wherein, the EtNBS fluorophores are (Z)-5-((9-(diethylamino)-5H-benzo[a]phenothiazin-5-ylidene)amino)pentanoic acid (EtNBS-COOH) derived fluorophores;
- (b) light-activating the standard and the test compositions to produce a fluorescence signal;
- (c) quantifying the fluorescence signal with a detector to obtain result;
- (d) comparing the test result to the standard result;
- and
- (e) if the amount of the measured fluorescence in the test sample is less than the amount of measured fluorescence in the standard sample, then the β-lactamase secreting bacteria is determined to be susceptible to the β-lactam antibiotic.
7 more elements
Dependencies are inferred from the claim text.