Microorganism having carbon dioxide fixation cycle introduced thereinto

A method of producing glutamic acid, comprising:

• culturing an acetyl-CoA producing microorganism comprising an acetyl-CoA production cycle obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e) to produce acetyl CoA:
• (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde catalyzed by malonate semialdehyde dehydrogenase, or an enzymatic reaction from malonyl-CoA to 3-hydroxypropionate catalyzed by malonyl-CoA reductase;
• (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate catalyzed by pyruvate synthase;
• (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA catalyzed by crotonyl-CoA carboxylase-reductase, or an enzymatic reaction from crotonyl-CoA and CO2 to glutaconyl-CoA catalyzed by methylcrotonyl-CoA carboxylase;
• (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate catalyzed by formate dehydrogenase;
• or
• (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase,
• the acetyl-CoA producing microorganism being obtained without imparting any of (a), (b), (c), or (d) to the microorganism, or such that the microorganism exhibits none of the functions of (a), (b), (c), or (d) even if one or more of (a), (b), (c), or (d) is imparted thereto;
• supplying at least one selected from the group consisting of a carbonate ion or a bicarbonate ion with a total supply amount of 150 mmol/L or more, and carbon dioxide gas with an average bubble diameter of 100 μm or more, to a culture medium used for the culturing and
• allowing the acetyl-CoA producing microorganism to produce glutamic acid, using the acetyl CoA.

A method of producing acetyl-CoA comprising:

• culturing an acetyl-CoA producing microorganism comprising an acetyl-CoA production cycle obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e):
• (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde catalyzed by malonate semialdehyde dehydrogenase, or an enzymatic reaction from malonyl-CoA to 3-hydroxypropionate catalyzed by malonyl-CoA reductase;
• (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate catalyzed by pyruvate synthase;
• (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA catalyzed by crotonyl-CoA carboxylase-reductase, or an enzymatic reaction from crotonyl-CoA and CO2 to glutaconyl-CoA catalyzed by methylcrotonyl-CoA carboxylase;
• (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate catalyzed by formate dehydrogenase;
• or
• (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase,
• the acetyl-CoA producing microorganism being obtained without imparting any of (a), (b), (c), or (d) to the microorganism, or such that the microorganism exhibits none of the functions of (a), (b), (c), or (d) even if one or more of (a), (b), (c), or (d) is imparted thereto;
• and
• supplying sodium sulfite with a total supply amount of from 0.01 g/l to 50 g/L to a culture medium used for the culturing wherein the microorganism produces acetyl-CoA.