Methods and compositions for the specific inhibition of glycolate oxidase (HAO1) by double-stranded RNA

A double stranded nucleic acid (dsNA) comprising first and second nucleic acid strands, wherein said first strand is 15-66 nucleotides in length and said second strand of said dsNA is 19-66 nucleotides in length, wherein said second strand is complementary to SEQ ID NO:

• 1823 along at least 19 consecutive nucleotides of said second oligonucleotide strand length and reduces HAO1 target mRNA expression when said double stranded nucleic acid is introduced into a mammalian cell, wherein the dsNA comprises one or more modified nucleotides.

A double stranded nucleic acid (dsNA) comprising first and second nucleic acid strands and a duplex region of at least 25 base pairs, wherein said first strand is 25-65 nucleotides in length and said second strand of said dsNA is 26-66 nucleotides in length and comprises 1-5 single-stranded nucleotides at its 3′ terminus, wherein said second strand is complementary to SEQ ID NO:

• 1823 along at least 19 consecutive nucleotides of said second oligonucleotide strand length and reduces HAO1 target gene expression when said double stranded nucleic acid is introduced into a mammalian cell, wherein the dsNA comprises one or more modified nucleotides.

A double stranded nucleic acid (dsNA) comprising first and second nucleic acid strands and a duplex region of at least 25 base pairs, wherein said first strand is 25-65 nucleotides in length and said second strand of said dsNA is 26-66 nucleotides in length and comprises 1-5 single-stranded nucleotides at its 3′ terminus, wherein the 3′ terminus of said first strand and the 5′ terminus of said second strand form a blunt end, and said second strand is complementary to SEQ ID NO:

• 1823 along at least 19 nucleotides of said second oligonucleotide strand length and reduces HAO1 mRNA expression when said double stranded nucleic acid is introduced into a mammalian cell, wherein the dsNA comprises one or more modified nucleotides.

A dsNA comprising a first strand and a second strand, wherein the first strand and the second strand form a duplex region of 19-25 nucleotides in length, wherein the first strand comprises a 3′ region that extends beyond the first strand-second strand duplex region and comprises a tetraloop, and the dsNA further comprises a discontinuity between the 3′ terminus of the first strand and the 5′ terminus of the second strand, and the first or second strand is complementary to SEQ ID NOs:

• NO:
• 1823 along at least 19 consecutive nucleotides of the first or second strand length and reduces HAO1 target mRNA expression when the dsNA is introduced into a mammalian cell, wherein the dsNA comprises one or more modified nucleotides.