Methods and compositions for producing active vitamin K-dependent proteins
AssigneeThe University of North Carolina at Chapel Hill
InventorsDarrel W. Stafford, Tao Li
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Abstract
The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
Claims10
A stable cell line overexpressing a recombinant vitamin Independent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the stable cell line is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the stable cell line in the absence of the recombinant VKOR and the recombinant VKGC.
A stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the stable cell line is increased 70% as compared to the amount of fully carboxylated VKD protein produced in the stable cell line in the absence of the recombinant VKOR and the recombinant VKGC.
A stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the stable cell line is increased 90% as compared to the amount of fully carboxylated VKD protein produced in the stable cell line in the absence of the recombinant VKOR and the recombinant VKGC.
Dependencies are inferred from the claim text.