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US 9,828,576 B2Granted
Cell culture
AssigneesNational University of Singapore, Centre National De La Recherche Scientifique
InventorsVirgile Nicolas Robert Viasnoff, Qiushi Li
Life of this patent
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Abstract
The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells.
Claims20
A cell culture vessel comprising a three dimensional cell culture substrate comprising:
- a perforated membrane with through holes,
- the perforated membrane having three membrane surfaces, namely an upper surface, a lower surface opposite to the upper surface, and a plurality of inner surfaces inside the through holes,
- the perforated membrane comprising a cured polymer adapted for cell culture having at least two modified cell culture surfaces among the three surfaces of the perforated membrane,
- wherein the first cell culture surface comprises at least one first cell culture agent for enhancing the proliferation and/or differentiation or function of the cells and the second cell culture surface does not comprise the first cell culture agent but a second different cell culture agent for enhancing the proliferation and/or differentiation or function of the cells,
- the first and the second cell culture surfaces being two surfaces out of the three membrane surfaces.
2 more elements
Dependencies are inferred from the claim text.