US 9,827,263 B2 · 2′-methoxy substituted oligomeric compounds and compositions for use in gene modulations

Abstract

Compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capable of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes a modified sugar and/or backbone modification. In some embodiments the modification is a 2′-OCH3 substituent group on a sugar moiety. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleotide of the oligomer has a modified sugar and/or backbone modification.

Claims20

Dependent claims

A composition comprising a first oligomer and a second oligomer, wherein:

at least a portion of said first oligomer is capable of hybridizing with at least a portion of said second oligomer,
at least a portion of said first oligomer is complementary to and capable of hybridizing with a selected target nucleic acid,
said first oligomer is a chimeric oligomeric compound,
wherein the chimeric oligomeric compound has an internal segment comprising five consecutive nucleosides having a 2′-OCH3 substituent group, and
wherein the chimeric oligomeric compound is not a gapmer.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/690,120Filed 17 Apr 2015 · continuation

Priority

5 Nov 2002

Published

US 2015/0366894 A124 Dec 2015

Granted

28 Nov 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

A61K 31/713
Compounds having three or more nucleosides or nucleotides: double-stranded nucleic acids or oligonucleotides
C12N 15/111
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: general methods applicable to biologically…
C12N 2310/14
Type of nucleic acid: interfering nucleic acids [NA]
C12N 2310/315
Of the backbone: phosphorothioates

Family

Application60/432,760Us provisional application · filed 5 Nov 2002

Application60/503,521Us provisional application · filed 16 Sep 2003

Application10/701,264Parent of this continuation · filed 4 Nov 2003

US 2015/0366894 A1Pre-grant publication · published 24 Dec 2015

US 2004/0147022 A1Published application

US 9,827,263 B2This patent

From the US filing history. Foreign family members aren’t listed.

2′-methoxy substituted oligomeric compounds and compositions for use in gene modulations

Life of this patent

2′-methoxy substituted oligomeric compounds and compositions for use in gene modulations

Life of this patent

Abstract

Claims20