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US 9,822,385 B2Granted
Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein
AssigneeAjinomoto Co., Inc.
InventorsYoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
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Abstract
An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
Claims6
A method for producing an acidic substance having a carboxyl group comprising
- culturing in a medium a microorganism, having an ability to produce the acidic substance having a carboxyl group, to produce and accumulate the acidic substance having a carboxyl group in the medium,
- collecting the acidic substance having a carboxyl group from the medium,
- wherein the acidic substance is L-glutamic acid and/or L-aspartic acid,
- wherein the microorganism has been modified to enhance expression of an ybjL gene, and
- wherein the ybjL gene encodes a protein selected from the group consisting of:
- (A) a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- and
- (B) a protein comprising a variant of the amino acid sequence of SEQ ID NO:
- 2, wherein one to 5 amino acid residues are substituted, deleted, inserted or added, and wherein, upon expression, said variant improves the ability of the microorganism to produce the acidic substance having a carboxyl group.
7 more elements
Dependencies are inferred from the claim text.