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US 9,822,384 B2Granted
Production of cannabinoids in yeast
AssigneeLibrede Inc.
InventorsJason L. Poulos, Anthony N. Farnia
Life of this patent
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Abstract
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Claims3
A method for making cannabigerolic acid, the method comprising:
- transforming S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.2 expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- and
- transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.13 expressing olivetolic synthase, expressing olivetolic acid cyclase and expressing aromatic prenyltransferase.
A method for making cannabigerolic acid, the method comprising:
- transforming a S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.1 expressing an acyl-activating enzyme;
- transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.22 expressing a mutant prenyltransferase;
- transforming the S. cervisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.10 expressing olivetolic synthase;
- transforming the S. cervisiae with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.5 expressing olivetolic acid cyclase;
- and
- transforming the S. cervisiae with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.13 expressing aromatic prenyltransferase.
3 more elements
A method for making cannabigerolic acid, the method comprising:
- transforming S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.23 expressing an acyl-activating enzyme;
- transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.24 expressing a mutant prenyltransferase;
- transforming the S. cervisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.25 expressing olivetolic synthase;
- transforming the S. cervisiae with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.26 expressing olivetolic acid cyclase;
- and
- transforming the S. cervisiae with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.27 expressing aromatic prenyltransferase.
3 more elements
Dependencies are inferred from the claim text.