Chimeric dengue virus E glycoproteins comprising mutant domain I and domain II hinge regions

A chimeric dengue virus E glycoprotein selected from the group consisting of:

• a) a first dengue virus E glycoprotein backbone from dengue virus serotype 2 (SEQ ID NO:20) comprising an A50V substitution, a K51T substitution, a Q52N substitution, a T55V substitution, a Y59L substitution, an N124K substitution, an M125L substitution, a V129I substitution, a P132Y substitution, an N203K substitution, an A205S substitution, an R210K substitution, an L221T substitution, a P222S, an M272T substitution, an S274G substitution, a G275T substitution, an N276T substitution, an L277T substitution, an L278I substitution, and a T280A substitution;
• b) a second dengue virus E glycoprotein backbone from dengue virus serotype 2 (SEQ ID NO:20) comprising a K51T substitution, a P53L substitution, a Y59L substitution, an N124P substitution, an M125I substitution, a P132Y substitution, an E202K substitution, an L207M substitution, an L214F substitution, an L221T substitution, a P222S substitution, an M272N substitution, an S274G substitution, an N276T substitution, an L277S substitution, an L278I substitution, and a I280A substitution;
• and
• c) a third dengue virus E glycoprotein backbone from dengue virus serotype 2 (SEQ ID NO:20) comprising an E49T substitution, a Q52E substitution, a P53V substitution, a T55L substitution, a K58T substitution, an N124K substitution, an M125I substitution, an E126T substitution, a K128N substitution, a V129L substitution, a P132I substitution, an E202K substitution, an N203K substitution, an A205T substitution, an R210K substitution, an L221T substitution, a P222A substitution, a Q271D substitution, an M272S substitution, an S273G substitution, an S274D substitution, an L277H substitution, an L278M substitution, and a T280A substitution.

A chimeric dengue virus E glycoprotein selected from the group consisting of:

• a) a first dengue virus E glycoprotein backbone from dengue virus serotype 3 (SEQ ID NO:17) comprising an A50V substitution, a Q52N substitution, an L53P substitution, a T55V substitution, a P124K substitution, an I125L substitution, a V129I substitution, a K200E substitution, an N201K substitution, an A203S substitution, an M205L substitution, an R208K substitution, an F212L substitution, an N270T substitution, a G273T substitution, and an S275T substitution;
• b) a second dengue virus E glycoprotein backbone from dengue virus serotype 3 (SEQ ID NO:17) comprising a T51K substitution, an L53P substitution, an L59Y substitution, a P124N substitution, an 125M substitution, a Y132P substitution, a K200E substitution, an M205L substitution, an F212L substitution, a T219L substitution, an S220P substitution, an N270M substitution, a G272S substitution, a T274N substitution, an S275L substitution, an I276L substitution, and an A278T substitution;
• c) a third dengue virus E glycoprotein backbone from dengue virus serotype 3 (SEQ ID NO:17) comprising an E49T substitution, a T51K substitution, a Q52E substitution, an L53V substitution, a T55L substitution, a K58T substitution, an L59Y substitution, a P124K substitution, an E126T substitution, a K128N substitution, a V129L substitution, a Y132I substitution, an N201K substitution, an A203T substitution, an M205L substitution, an R208K substitution, an F212L substitution, an S220A substitution, a Q269D substitution, an N270S substitution, an S271G substitution, a G272D substitution, a T274N substitution, an S275H substitution, and an I276M substitution;
• and
• d) a fourth dengue virus E glycoprotein backbone from dengue virus serotype 3 (SEQ ID NO:17) comprising a T51K substitution, a Q52E substitution, an L53V substitution, a T55L substitution, a K58T substitution, an L59Y substitution, a P124K substitution, an E126T substitution, a K128N substitution, a V129I, substitution, a T198K substitution, N201K substitution, an A203T substitution, an M205L substitution, an R208K substitution, an F212L substitution, an S220A substitution, an I268V substitution, a Q269D substitution, an N270S substitution, an S271G substitution, a G272D substitution, a T274N substitution, an S275H substitution, and an 1276M substitution.

A chimeric dengue virus E glycoprotein selected from the group consisting of:

• a) a first dengue virus E glycoprotein backbone from dengue virus serotype 4 (SEQ ID NO:19) comprising a T49E substitution, an A50V substitution, a K51T substitution, an E52N substitution, a VS3P substitution, an L55V substitution, a T58K substitution, a Y59L substitution, an I125L substitution, a T126E substitution, an N128K substitution, an L129I substitution, an I132Y substitution, a K202E substitution, a T205S substitution, an A222S substitution, a D271Q substitution, an S272T substitution, a G273S substitution, a D274G substitution, a G275T substitution, an N276T substitution, an H277T substitution, and an M278I substitution;
• b) a second dengue virus E glycoprotein backbone from dengue virus serotype 4 (SEQ ID NO:
• 19) comprising a T49E substitution, an E52Q substitution, a V53P substitution, an L55T substitution, a T58K substitution, a K124N substitution, an I125M substitution, a T126E substitution, an N28K substitution, an L129V substitution, an I132P substitution, a K202E substitution, a K203N substitution, a T205A substitution, a K210R substitution, a T221L substitution, an A222P substitution, a D271Q substitution, an S272M substitution, a G273S substitution, a D274S substitution, an H277L substitution, an M278L substitution, and an A280T substitution;
• and
• c) a third dengue virus E glycoprotein backbone from dengue virus serotype 4 (SEQ ID NO:19) comprising a T49E substitution, a K51T substitution, an E52Q substitution, a V53L substitution, an L55T substitution, a T58K substitution, a Y59L substitution, a K124P substitution, a T126E substitution, an N128K substitution, an L129V substitution, an I132Y substitution, a K203N substitution, a T205A substitution, an I207M substitution, a K210R substitution, an L214F substitution, an A222S substitution, a D271Q substitution, an S272N substitution, a G273S substitution, a D274G substitution, an N276T substitution, an H277S substitution, and an M278I substitution.