Device for detection of target molecules and uses thereof

A method of detecting a target molecule, the method comprising:

• contacting a sample with a device, the device comprising a housing, the housing comprising a first housing member, a second housing member, and an inlet opening,
• wherein the housing further comprises:
• a membrane detection system,
• wherein the membrane detection system comprises:
• a conjugate pad comprising a first capture reagent;
• a permeable membrane;
• a test membrane comprising a second capture reagent;
• and
• two absorbent members;
• a force member that applies pressure substantially perpendicular to the membrane detection system, wherein the vertical axis of the force member is substantially perpendicular to the membrane detection system;
• a slidable locking member contacting the force member;
• an attachment member in contact with the slidable locking member;
• and
• a sliding button attached to the slidable locking member,
• wherein the conjugate pad, the permeable membrane, the test membrane;
• and the two absorbent members are substantially parallel to each other and the inlet opening,
• wherein the inlet opening is in fluid contact with the membrane detection system,
• wherein the slidable locking member moves the conjugate pad when the slidable locking member is moved, and
• wherein the conjugate pad is operably connected to the slidable locking member and the conjugate pad is moved by moving the slidable locking member,
• wherein the sample contacted with the membrane detection system flows vertically from the conjugate pad to the test membrane;
• moving the conjugate pad after a portion of the sample has contacted and flowed through the conjugate pad, thereby exposing at least a portion of the test membrane for detection;
• and
• identifying a positive or negative reaction for the target molecule.