Chromatography media and method
AssigneeEMD Millipore Corporation
InventorsDavid Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
Life of this patent
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Abstract
Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).
Claims15
A process for purifying a sample comprising a biomolecule, comprising contacting said sample with a bed of fiber media, said fibers having a cross-section comprising a body region defining a substantially longitudinal axis, and having a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling ion-exchange chromatography.
A process of purifying a sample comprising a biomolecule of interest and impurities, comprising:
- providing a sample comprising the biomolecule of interest and impurities;
- contacting said sample with a chromatography media comprising an axially compressed bed of cut nylon staple fibers, said fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon anion-exchange functionality, acrylic polymer functionality or cation-exchange functionality thereby to bind the biomolecule of interest or impurities;
- and
- recovering the biomolecule of interest.
A process for purifying a negatively-charged virus, comprising providing a sample containing negatively-charged virus;
- contacting said sample containing said negatively-charged virus with an axially compressed bed of cut nylon staple fiber media, wherein said cut fibers have a cross-section comprising a body region defining a substantially longitudinal axis, and have a plurality of projections extending outwardly from said body region, and wherein said fibers have imparted thereon a polymeric functionality, wherein the surfaces of said polymeric functionality are modified with pendant trimethylammonium groups;
- washing said fibers to remove unbound species;
- and eluting said negatively-charged virus, thereby to purify the negatively-charged virus;
- wherein said negatively charged virus is bacteriophage ø6.
A process for removing a negatively-charged virus from a sample, comprising providing a sample containing negatively-charged virus;
- contacting said sample containing said negatively-charged virus with an axially compressed bed of cut nylon staple fiber media, wherein said cut fibers have a cross-section comprising a body region defining a substantially longitudinal axis, and have a plurality of projections extending outwardly from said body region, said fibers having imparted thereon a polymeric functionality, wherein the surfaces of said polymeric functionality are modified with pendant trimethylammonium groups, thereby binding said negative-charged virus to said fibers, washing said fibers to remove unbound species;
- and eluting said negatively-charged virus;
- wherein said negatively charged virus is bacteriophage ø6.
Dependencies are inferred from the claim text.