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US 9,809,862 B2Granted
G-protein coupled receptor (GPCR)-based biosensors and uses thereof
AssigneeGeorgia Tech Research Corporation
InventorsPamela Peralta-Yahya, Kuntal Mukherjee, Souryadeep Bhattacharyya, Stephen Sarria
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Abstract
Provided herein are GPCR-based chemical biosensors that can have a sensing unit, a processing unit, and a response unit that can be used to detect a chemical of interest. Also provided herein are methods of making and using the GPCR-based chemical biosensors.
Claims20
An engineered yeast cell comprising:
- a sensing unit, the sensing unit comprising:
- a G-protein coupled receptor (GPCR);
- a synthetic processing unit, the synthetic processing unit comprising:
- a signal transduction pathway, wherein the signal transduction pathway comprises a synthetic transcription factor selected from the group consisting of STF1, STF2, SFT3 and combinations thereof;
- and
- a synthetic response unit, the synthetic response unit comprising:
- a recombinant signal molecule gene, where the recombinant signal molecule gene is operatively coupled to a synthetic promoter that is responsive to the synthetic processing unit, wherein the synthetic promoter is selected from the group consisting of:
- pGAL45x, pLExA4x, pCRE, and combinations thereof,
- wherein the sensing unit is in biologic communication with the processing unit and the processing unit is in biologic communication with the response unit.
6 more elements
A method comprising:
- contacting an engineered yeast cell with a sample, wherein the yeast cell comprises
- a sensing unit, the sensing unit comprising:
- a G-protein coupled receptor (GPCR);
- a synthetic processing unit, the synthetic processing unit comprising:
- a signal transduction pathway, wherein the signal transduction pathway comprises a synthetic transcription factor selected from the group consisting of STF1, STF2, SFT3 and combinations thereof;
- and
- a synthetic response unit, the synthetic response unit comprising:
- a recombinant signal molecule gene, wherein the recombinant signal molecule gene is operatively coupled to a synthetic promoter that is responsive to the synthetic processing unit, wherein the synthetic promoter is selected from the group consisting of:
- pGAL45x, pLExA4x, pCRE, and combinations thereof,
- wherein the sensing unit is in biologic communication with the processing unit and the processing unit is in biologic communication with the response unit.
7 more elements
Dependencies are inferred from the claim text.