Method of evaluating inhibitory effect on damp-dry malodor

A method of screening a damp-dry malodor inhibitor, which comprises

• bringing microorganisms which produce damp-dry malodor-causing substances and a test substance into contact with each other in the presence of a sebaceous dirt component;
• detecting expression of at least one gene selected from the group consisting of a fatty acid desaturase gene and a β oxidation-related enzyme gene derived from the microorganisms;
• and thereby
• selecting or evaluating a test substance that reduces the expression amount of the at least one gene as a damp-dry malodor inhibitor,
• wherein the expression of the at least one gene is detected by using at least one oligonucleotide pair selected from the group consisting of:
• an oligonucleotide pair containing oligonucleotides (a) and (b) as described below,
• an oligonucleotide pair containing oligonucleotides (c) and (d) as described below,
• an oligonucleotide pair containing oligonucleotides (e) and (f) as described below,
• an oligonucleotide pair containing oligonucleotides (g) and (h) as described below,
• an oligonucleotide pair containing oligonucleotides (i) and (j) as described below,
• an oligonucleotide pair containing oligonucleotides (k) and (l) as described below,
• an oligonucleotide pair containing oligonucleotides (m) and (n) as described below,
• an oligonucleotide pair containing oligonucleotides (o) and (p) as described below,
• an oligonucleotide pair containing oligonucleotides (q) and (r) as described below,
• an oligonucleotide pair containing oligonucleotides (s) and (t) as described below,
• an oligonucleotide pair containing oligonucleotides (u) and (v) as described below,
• an oligonucleotide pair containing oligonucleotides (w) and (x) as described below,
• an oligonucleotide pair containing oligonucleotides (y) and (z) as described below,
• an oligonucleotide pair containing oligonucleotides (a1) and (b1) as described below, and
• an oligonucleotide pair containing oligonucleotides (c1) and (d1) as described below:
• wherein
• oligonucleotide (a) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 13, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 13 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (b) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 14, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 14 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (c) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 15, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 15 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (d) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 16, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 16 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (e) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 17, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 17 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (f) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 18, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 18 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (g) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 19, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 19 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (h) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 20, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 20 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (i) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 21, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 21 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (j) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 22, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 22 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (k) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 23, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 23 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 24, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 24 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (m) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 25, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 25 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (n) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 26, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 26 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (o) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 27, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 27 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (p) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 28, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 28 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (q) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 29, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 29 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (r) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 30, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 30 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (s) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 31, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 31 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (t) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 32, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 32 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (u) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 33, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 33 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (v) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 34, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 34 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (w) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 35, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 35 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (x) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 36, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 36 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (y) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 37, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 37 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (z) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 38, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 38 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (a1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 39, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 39 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (b1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 40, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 40 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (c1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 41, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 41 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• and
• oligonucleotide (d1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 42, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 42 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene.

A method of detecting microorganisms which produce damp-dry malodor-causing substances, comprising the steps of:

• amplifying at least one fragment of a gene selected from the group consisting of a Δ9desaturase gene, an fadB gene and an fadD gene derived from microorganisms which produce damp-dry malodor-causing substances;
• determining the presence or absence of an amplified fragment;
• and thereby
• detecting the microorganisms which produce damp-dry malodor-causing substances,
• wherein the detection of the microorganisms which produce damp-dry malodor-causing substances is performed by using at least one oligonucleotide pair selected from the group consisting of:
• an oligonucleotide pair containing oligonucleotides (c) and (d) as described below,
• an oligonucleotide pair containing oligonucleotides (e) and (f) as described below,
• an oligonucleotide pair containing oligonucleotides (g) and (h) as described below,
• an oligonucleotide pair containing oligonucleotides (i) and (j) as described below,
• an oligonucleotide pair containing oligonucleotides (k) and (l) as described below,
• an oligonucleotide pair containing oligonucleotides (m) and (n) as described below,
• an oligonucleotide pair containing oligonucleotides (o) and (p) as described below,
• an oligonucleotide pair containing oligonucleotides (q) and (r) as described below,
• an oligonucleotide pair containing oligonucleotides (s) and (t) as described below,
• an oligonucleotide pair containing oligonucleotides (u) and (v) as described below,
• an oligonucleotide pair containing oligonucleotides (w) and (x) as described below,
• an oligonucleotide pair containing oligonucleotides (y) and (z) as described below,
• an oligonucleotide pair containing oligonucleotides (a1) and (b1) as described below, and
• an oligonucleotide pair containing oligonucleotides (c1) and (d1) as described below:
• wherein
• oligonucleotide (c) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 15, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 15 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (d) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 16, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 16 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (e) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 17, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 17 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (f) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 18, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 18 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (g) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 19, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 19 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (h) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 20, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 20 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (i) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 21, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 21 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (j) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 22, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 22 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (k) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 23, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 23 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (l) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 24, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 24 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (m) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 25, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 25 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (n) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 26, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 26 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (o) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 27, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 27 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (p) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 28, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 28 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (q) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 29, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 29 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (r) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 30, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 30 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (s) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 31, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 31 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (t) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 32, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 32 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (u) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 33, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 33 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (v) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 34, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 34 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (w) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 35, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 35 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (x) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 36, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 36 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (y) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 37, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 37 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (z) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 38, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 38 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (a1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 39, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 39 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (b1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 40, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 40 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (c1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 41, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 41 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• and
• oligonucleotide (d1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 42, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 42 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene.

A kit for evaluating an inhibitory effect on damp-dry malodor or a kit for screening for a damp-dry malodor inhibitor, comprising:

• microorganisms that produce damp-dry malodor-causing substances due to expression of the microorganisms' Δ9desaturase gene, fadB gene or fadD gene;
• a sebaceous dirt component;
• and
• an oligonucleotide selected from the group consisting of the following oligonucleotides (c) to (z) and (a1) to (d1);
• or an oligonucleotide pair selected from the group consisting of:
• a pair of the following oligonucleotides (c) and (d),
• a pair of the following oligonucleotides (e) and (f),
• a pair of the following oligonucleotides (g) and (h),
• a pair of the following oligonucleotides (i) and (j),
• a pair of the following oligonucleotides (k) and (l),
• a pair of the following oligonucleotides (m) and (n),
• a pair of the following oligonucleotides (o) and (p),
• a pair of the following oligonucleotides (q) and (r),
• a pair of the following oligonucleotides (s) and (t),
• a pair of the following oligonucleotides (u) and (v),
• a pair of the following oligonucleotides (w) and (x),
• a pair of the following oligonucleotides (y) and (z), a pair of the following oligonucleotides (a1) and (b1), and
• a pair of the following oligonucleotides (c1) and (d1):
• wherein
• oligonucleotide (c) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 15, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 15 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (d) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 16, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 16 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (e) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 17, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 17 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (f) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 18, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 18 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (g) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 19, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 19 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (h) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 20, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 20 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (i) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 21, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 21 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (j) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 22, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 22 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (k) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 23, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 23 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (l) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 24, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 24 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the fatty acid desaturase gene;
• oligonucleotide (m) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 25, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 25 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (n) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 26, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 26 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (o) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 27, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 27 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (p) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 28, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 28 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (q) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 29, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 29 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (r) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 30, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 30 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (s) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 31, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 31 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (t) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 32, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 32 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (u) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 33, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 33 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (v) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 34, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 34 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (w) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 35, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 35 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (x) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 36, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 36 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (y) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 37, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 37 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (z) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 38, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 38 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (a1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 39, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 39 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (b1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 40, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 40 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• oligonucleotide (c1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 41, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 41 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene;
• and
• oligonucleotide (d1) is an oligonucleotide having the base sequence set forth in SEQ ID NO:
• 42, or an oligonucleotide having a base sequence set forth in SEQ ID NO:
• 42 with a substitution, deletion, insertion, or addition of 1 to 3 nucleotides which can be used for detecting the β oxidation-related enzyme gene.