Test kits and methods for their use in detecting expression ratios of genetic markers

A test kit for detection of expression ratios of genetic markers for transitional cell carcinoma (TCC), comprising:

• a forward polymerase chain reaction (PCR) primer consisting of the sequence of SEQ ID NO. 1 and a reverse PCR primer consisting of the sequence of SEQ ID NO. 2 for hybridizing to an mRNA or Cy3 or Cy5 labeled cDNA transcript of insulin-like growth factor binding protein 5 (“IGFBP5”);
• a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe consisting of the sequence of SEQ ID NO. 3 that hybridizes to an mRNA or a cDNA transcript of said IGFBP5 between the binding locations of said forward and reverse primers of IGFBP5;
• a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or a cDNA oligonucleotide transcript oligonucleotide of homeobox A13 (“HOXA13”);
• a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 Labeled cDNA transcript of said HOXA13 between the binding locations of said forward and reverse primers of HOXA13;
• a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or cDNA transcript of midkine (neurite growth promoting factor 2 (“MDK”);
• a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 labeled cDNA transcript of said MDK between the binding locations of said forward and reverse primers of MDK;
• a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or Cy3 or Cy5 labeled cDNA transcript of leukotriene B4 12-dehydrogenase (“LTB4DH”);
• and
• a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 labeled cDNA transcript of said LTB4DH between the binding locations of said forward and reverse primers of LTB4DH.