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US 9,809,857 B2Granted
Methods and signatures for oropharyngeal cancer prognosis
AssigneeWashington University
InventorXiaowei Wang
Life of this patent
Estimating the term…
Abstract
Methods for determining the prognosis of a subject with oropharyngeal squamous cell carcinoma are described, as well as miRNAs and panels of prognostic miRNAs that are used in the methods.
Claims18
A method of identifying a subject with oropharyngeal cancer as having a poor or a favorable prognosis, the method comprising:
- (a) obtaining a tissue sample yielding low quality RNA from a subject;
- (b) using a PCR-based profiling method for miRNA expression, to determine in the sample, the levels of six prognostic miRNAs selected from the group consisting of miR-142-3p, miR-31, miR-146a, miR-26b, miR-24, and miR-193b;
- (c) calculating a risk score using the levels of miRNAs determined, and
- (d) comparing the risk score to a median risk score,
- wherein, if (i) the risk score is equal to or higher than the median risk score, the subject has a poor prognosis, and (ii) the risk score is lower than the median risk score, the subject has a favorable prognosis, wherein poor prognosis is indicative of a significantly greater risk of death.
2 more elements
A method for determining the prognosis of a subject with oropharyngeal carcinoma, the method comprising:
- (a) obtaining a tissue sample yielding low quality RNA from a subject;
- (b) using a PCR-based profiling method for miRNA expression, to determine in the sample, the levels of six prognostic miRNAs selected from the group consisting of miR-142-3p, miR-31, miR-146a, miR-26b, miR-24, and miR-193b;
- (c) calculating a risk score (S) according to the following formula:
- S=C1E1 +C2E2+C3E3−C4E4−C5E5−C6E6, wherein:
- C1, C2, C3, C4, C5, and C6 are coefficients;
- E1 is the expression level of miR-24;
- E2 is the expression level of miR-31;
- E3 is the expression level of miR-193b;
- E4 is the expression level of miR-26b;
- E5 is the expression level of miR-142-3p;
- E6 is the expression level of miR-146a;
- and
- assessing prognosis by comparing the risk score to a median risk score, wherein, if (i) the risk score is equal to or higher than the median risk score, the subject has a poor prognosis, and (ii) the risk score is lower than the median risk score, the subject has a favorable prognosis, wherein poor prognosis is indicative of a significantly greater risk of death.
10 more elements
Dependencies are inferred from the claim text.