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US 9,809,846 B2Granted
Compositions, kits, uses and methods for amplified detection of an analyte
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsItamar Willner, Fuan Wang, Chun-Hua Lu, Xiaoqing Liu, Lina Freage
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Abstract
The invention provides compositions comprising rolling circle amplification sequences and hairpin sequences specifically designed for the accurate and highly sensitive detection of one or more analyte sequences. The invention further provides kits comprising them and methods for their use.
Claims20
A composition comprising:
- at least one hairpin selected from the group consisting of SEQ. ID. NO. 4 and SEQ. ID. NO. 8, the at least one hairpin sequence comprising
- at least one analyte sequence I′, and
- at least one sequence II;
- and
- at least one circular sequence selected from the group consisting of SEQ. ID. NO. 2, SEQ. ID. NO. 6 and SEQ. ID. NO. 9, the at least one circular sequence comprising
- at least one sequence I being complementary with sequence I′,
- at least one sequence II, and
- at least one sequence III encoding a biosensor sequence III′.
5 more elements
A method of detecting at least one analyte sequence I′ comprising the steps of:
- contacting a sample with a composition comprising at least one hairpin sequence selected from the group consisting of SEQ. ID. NO. 4 and SEQ. ID. NO. 8;
- said at least one hairpin sequence comprising at least one analyte sequence I′;
- and at least one sequence II;
- and at least one circular sequence selected from the group consisting of SEQ. ID. NO. 2, SEQ. ID. NO. 6 and SEQ. ID. NO. 9;
- wherein said at least one circular sequence comprising:
- at least one sequence I being complementary with sequence I′;
- at least one sequence II;
- and at least one sequence III encoding a biosensor sequence III′;
- thereby forming a mixture of said sample and composition;
- detecting the biosensor sequence III′ in said mixture;
- thereby detecting said sequence I′.
8 more elements
A method of detecting at least one analyte sequence I′ and/or at least one analyte sequence IV′ comprising the steps of:
- contacting a sample with a composition comprising at least one hairpin sequence comprising at least one analyte sequence I′;
- and at least one sequence II;
- at least one further hairpin sequence comprising:
- at least one analyte sequence IV′;
- and at least one sequence V;
- at least one circular sequence comprising:
- at least one sequence I being complementary with sequence I′;
- at least one sequence II;
- and at least one sequence III encoding a biosensor sequence III′;
- and at least one further circular sequence comprising:
- at least one sequence IV being complementary with sequence IV′;
- at least one sequence V;
- and at least one sequence VI encoding a biosensor sequence VI′;
- thereby forming a mixture of said sample and composition;
- detecting the biosensor sequence III′ and/or biosensor sequence VI′ in said mixture;
- thereby detecting said sequence I′ and/or sequence IV′,
- wherein each of the at least one hairpin sequence and the at least one further hairpin sequence is selected from the group consisting of SEQ. ID. NO. 4 and SEQ. ID. NO. 8, and
- wherein each of the at least one circular sequence and at least one further circular sequence is selected from the group consisting of SEQ. ID. NO. 2, SEQ. ID. NO. 6 and SEQ. ID. NO. 9.
15 more elements
Dependencies are inferred from the claim text.