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US 9,809,838 B2Granted
Methods for determining the concentration of an analyte in solution
AssigneeTrustees of Tufts College
InventorsDavid R. Walt, David M. Rissin
Life of this patent
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Abstract
Disclosed is a method for measuring the concentration of an analyte or analytes in a solution. Although the methods can be conducted using a number of different assay formats, in one embodiment, the assays are conducted in reaction vessels defined, at least in part, by the distal ends of fiber optic strands.
Claims10
A method of determining the concentration of an analyte in a fluid sample to be tested, the method comprising the steps of:
- (a) partitioning at least a portion of the analyte molecules in the fluid sample across a plurality of reaction vessels so that more than 20% but less than 95% of the reaction vessels contain at least one molecule, wherein the analyte is attached to a nanoparticle or microparticle by a capture component;
- (b) determining the presence or absence of the analyte in each reaction vessel to identify the number of reaction vessels that contain analyte and/or to identify the number of reaction vessels that contain no analyte;
- and
- (c) determining the concentration of the analyte in the fluid sample by a Gaussian distribution analysis of the number of reaction vessels that contain the analyte.
1 more element
Dependencies are inferred from the claim text.