Pathways to adipate semialdehyde and other organic products
AssigneeBioamber S.A.S.
InventorsMan Kit Lau, Christopher P. Mercogliano
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Abstract
Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided.
Claims11
A recombinant microorganism genetically engineered to produce adipate semialdehyde from 4-hydroxybutyryl-CoA, the recombinant microorganism comprising a first exogenous nucleic acid encoding a CoA synthase of class E.C. 2.3.1.X that catalyzes a substrate to product conversion of malonyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO2, wherein the recombinant microorganism produces adipate semialdehyde from 4-hydroxybutyryl-CoA via the intermediates:
- 6-hydroxy-3-oxo-hexanoyl-CoA;
- 3,6-dihydroxyhexanoyl-CoA;
- 6-hydroxy-2-hexenoyl-CoA;
- 6-hydroxyhexanoyl-CoA;
- and 6-hydroxycaproic acid.
A recombinant microorganism genetically engineered to produce adipate semialdehyde from 4-hydroxybutyryl-CoA, the recombinant microorganism comprising a first exogenous nucleic acid encoding an enzyme of class E.C. 2.3.1.165, E.C. 2.3.1.194, E.C. 2.3.1.199, E.C. 2.3.1.206, or E.C. 2.3.1.221 that catalyzes a substrate to product conversion of malonyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO2, and wherein the recombinant microorganism produces adipate semialdehyde from 4-hydroxybutyryl-CoA via the intermediates:
- 6-hydroxy-3-oxo-hexanoyl-CoA;
- 3,6-dihydroxyhexanoyl-CoA;
- 6-hydroxy-2-hexenoyl-CoA;
- 6-hydroxyhexanoyl-CoA;
- and 6-hydroxycaproic acid.
Dependencies are inferred from the claim text.