Mutant NNK1 allele and its use
AssigneesVib Vzw, Katholieke Universiteit Leuven, KU LEUVEN R&D
InventorsJohan Thevelein, Mekonnen Demeke, Maria Remedios Foulquié Moreno
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Abstract
The present invention relates to a mutant NNK1 allele, especially a mutant carrying a mutation at position of amino acid 807 of the wild type sequence. The invention relates further to the use of said mutant allele to increase the fermentation rate in yeast, preferably in Saccharomyces. The mutant allele is especially useful to increase the xylose to ethanol fermentation rate.
Claims18
A genetically modified xylose fermenting yeast strain, comprising a mutant DNA molecule that corresponds to a mutant nitrogen network kinase 1 (NNK1) allele and encodes a mutant kinase protein carrying a mutation at position 807 of a wild type kinase protein the amino acid sequence of which is 95% identical to SEQ ID NO:
- 2, wherein the yeast strain is not Saccharomyces cerevisiae strain GS1.11-26 and wherein the mutant increases the xylose-to-ethanol fermentation rate as compared to the wild type.
Dependencies are inferred from the claim text.