Compositions and methods for efficacious and safe delivery of siRNA using specific chitosan-based nanocomplexes
AssigneePolyvalor, Limited Partnership
InventorsAbderrazzak Merzouki, Michael D. Buschmann
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Abstract
There is disclosed a composition and a method for the efficient delivery of a therapeutic RNAi-inducing nucleic acid to cells both in vitro and in vivo through specific formulations of a non viral delivery system using chitosans. Particularly, the composition contains a nucleic acid and a specific chitosan that has the following physico-chemical properties: a number-average molecular weight between 5 kDa and 200 kDa, a degree of deacetylation between 80% and 95% and a chitosan amine to nucleic acid phosphate ratio below 20.
Claims24
A method for in vivo delivery of an RNAi-inducing nucleic acid molecule in a patient, the method comprising:
- administering to said patient a composition comprising chitosan and an RNAi-inducing nucleic acid molecule, wherein the RNAi-inducing nucleic acid molecule is a short interfering RNA (siRNA) or a short hairpin RNA (shRNA), wherein the chitosan has a molecular weight (Mn) of 5 kDa to 200 kDa, a degree of deacetylation (DDA) of 80% to 95%, wherein the chitosan amine to nucleic acid phosphate ratio (N:P) is below 20.
Dependencies are inferred from the claim text.