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US 9,809,811 B2Granted
Method of random circular permutation by MuCP-ISC and MuCP-ISSC transposons
AssigneeNew York University
InventorsJin Ryoun Kim, Brennal Pierre, Vandan Shah
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Abstract
A method for designing circular permuted proteins using two engineered Mu transposons for easy construction of random circular permuted proteins, the two designed transposons being MuCP-ISC (Mu Circular permutation transposon with Integrated Start Codon) and MuCP-ISSC (Mu Circular permutation transposon with Integrated Start and Stop Codon).
Claims27
A method for designing circular permuted proteins, comprising constructing random circular permuted proteins using two engineered Mu transposons.
A method for designing circular permuted proteins, comprising:
- a) constructing random circular permuted proteins using a Mu Circular permutation transposon (MuCP);
- b) restricting the length of the appended amino acids to 1 at N-terminus for the MuCP and to 2 or 3 at C-terminus for the MuCP;
- c) comprising giving flexibility to the length of the linker sequence connecting the N and C termini for the MuCP.
Dependencies are inferred from the claim text.