Method for producing parvovirus having high infectivity titer
AssigneeAsahi Kasei Medical Co., Ltd.
InventorKoichiro Yanagida
Life of this patent
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Abstract
A method for stably and easily producing a parvovirus having a high infectivity titer is provided. The problem is solved by a method for producing a parvovirus having an infectivity titer as high as 108 TCID50/mL or more in a culture supernatant, comprising the steps of inoculating a seed virus of the parvovirus into a culture substrate comprising host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown and a medium at a multiplicity of infection of 0.0001 to 0.1, culturing for a period of 5 to 11 times the doubling time of the host cells, and recovering a culture supernatant.
Claims15
A method for producing a parvovirus in a culture supernatant by culturing host cells and a seed virus of the parvovirus in a culture substrate, comprising:
- (a) calculating:
- a doubling time of the host cells during the log growth phase in the culturing;
- and a cell density of the host cells confluently grown in the culturing;
- (b) inoculating the seed virus of the parvovirus into the culture substrate comprising a medium and the host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown, calculated in (a), to give a multiplicity of infection (MOI) of 0.0001 to 0.1;
- (c) culturing the culture comprising the medium and the host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown and the inoculated parvovirus obtained in (b) for a period of 5 to 11 times the doubling time calculated in (a);
- and
- (d) recovering a culture supernatant comprising the parvovirus, obtained by the culturing (c).
Dependencies are inferred from the claim text.